Plant Genetic Transformation using Tissue Culture

Genetic transformation is a technique used to transfer foreign DNA into plant genomes. Usually Agrobacterium tumefaciens or direct DNA uptake methods like biolistics are employed.
Plant Tissue Culture techniques provide an ideal platform for regeneration of transformed plants. Suitable explants are infected or bombarded with transforming DNA and then cultured in special media selective for transformed cells. Only transformed cells survive and regenerate into transgenic shoots. Proper screening and analysis confirms stable integration and expression of transgenes in the regenerants. This method is important for developing crop varieties with agronomically useful traits like herbicide, pest or disease resistance.
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